Extracellular Vesicle (EV) heterogeneity is seen both within and between species. In both eukaryotes and prokaryotes, these can be categorised as Micro and Nano-EVs (often exosomes in mammals), linked to their many mechanisms of biogenesis. Fundamentally, the methodologies for isolation and characterisation of EVs, irrespective of their species of origin can be standardised. I will present our work on the isolation, characterisation and functional analyses of placenta-derived and bacterial EVs, particularly with a focus on their nucleic acid cargos. Then I will present how we have brought these and other seemingly diverse research subjects together to establish a “Hub for Extracellular Vesicle Investigations”, the first dedicated EV research centre in New Zealand.