Poster Presentation Australasian Extracellular Vesicles Conference 2018

Analysis of Extracellular Vesicles derived-microRNAs from Malaria patients' plasma  (#99)

Nutpakal Ketprasit 1 2 , Iris Cheng 2 , Valery Combes 2 , Duangdao Palasuwan 1
  1. Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, -, Thailand
  2. School of Life Sciences, Faculty of Sciences, University Technology of Sydney, Sydney, New South Wales, Australia

             Malaria is one of the most important parasitic disease. There were an estimated 438,000 malaria deaths worldwide. Nowadays, the disease remains major challenge as drug resistance malaria parasites were emerged especially in Plasmodium falciparum where P.vivax malaria incidence is mounting. Understanding both pathogenesis and drug resistance mechanism are thus crucial for improving malaria elimination. 
             Extracellular vesicles(EVs), a small membrane-bound vesicle, have been interested in malaria for a decade. The numbers of cells-derived EVs; red cell-derived mirovesicles (MVs), Endothelial cell-derived MVs and platelet-derived MVs increased during the infection. They play an important role in malaria pathogenesis both in human and mice malaria model. EVs also contribute in cell-cell communication. For example, exosomes like EVs promoted gametocyte formation or they induced transcriptional change in human monocyte in vitro. Recently, there were studies showed that EVs also cargo microRNAs and could modulate host cells in vitro. but there is no information about EVs derived-miRNAs in human malaria samples.
            Here, we showed the methods for studied and evaluated EVs derived-miRNAs from malaria patient plasma comparing with healthy donor plasma. Total RNA samples were extracted from isolated EVs and were reverse transcribed to complementary DNA. Taqman assay was used to analyze miRNAs expression. The result showed that there was no different expression of has-mir-let7 in both P.falciparum(n=11)and P.vivax(n=11)infected plasma comparing to healthy(n=11). For further study, we suggested for studying other miRNAs that are biological relevant to malaria infection in various aspects to improve knowledge about the disease.

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