Mesenchymal stem cells have an excellent potential in both curing different disease conditions and reducing cell damage because of different biochemical agents produced during different physiological processes. The objective of this study was to investigate the role of canine Adipose-derived mesenchymal stem cells (AT-MSCs) in the production of different proteins into the culture medium that plays a key role in cell protection and repair. Gene expression of different proteins associated with cell repair including Annexin I, Annexin II, Histone H2A and Vimentin was analyzed in AT-MSCs using reverse transcription polymerase chain reaction (RT-PCR), whereas the secretion of these proteins into the culture medium by AT-MSCs at different passage lines and incubation duration was tested by western blotting. Results demonstrated a positive expression level of genes in the canine AT-MSCs, along with secretion of the above-mentioned proteins in the culture medium. Results lead us to a conclusion that canine AT-MSCs can be helpful in reducing the cellular damage through secretion of different proteins in vivo and in vitro conditions.